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Download Advances in Applied Microbiology, Vol. 3 by Wayne W Umbreit PDF

By Wayne W Umbreit

ISBN-10: 0120026031

ISBN-13: 9780120026036

Meant for researchers in utilized microbiology and environmental engineers, this e-book covers such themes as environmental evaluate of biotechnological strategies and microbial alterations of haloaromatic and haloaliphatic compounds.

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Extra info for Advances in Applied Microbiology, Vol. 3

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Lovelock and Bishop believed that dimethylsulfoxide protected erythrocytes more than did glycerol because the cells were more permeable to dimethylsulfoxide than to glycerol. Cells were frozen at -79O and thawed at 35OC. Rates of temperature change were not given but they appear to have been relatively slow and good survival was obtained. , 1958). Nei (1960) studied the effects of freezing rate and lyophilization on a variety of microorganisms by measuring physiological activities of recovered cells.

By comparison, between 1 and 15%of the Vibrio he processed in the same manner remained viable after 9 to 12 months storage at room temperature. From the above discussion it seems that the differences among bacteria are as great or greater than the differences between bacteria and yeasts. Viruses are, as a whole, more sensitive to certain drying procedures, but they are more resistant to cold shock than are bacteria. Most rickettsiae are relatively difficult to maintain as dry stock cultures although Topping (1940) and Jackson and Smadel (1951) were successful in maintaining infectivity by lyophilization.

Stamp ( 1947), Fry and Greaves (1951), and others have added glucose (which is hygroscopic) to culture suspensions immediately before drying to assure some residual moisture. Muggleton (1960) contended that com- 44 ROBERT J. HECKLY plete removal of water is incompatible with life, at least with respect to the tubercle bacillus. He also added glucose to retain moisture but he did not show that the beneficial effect was due to increased moisture rather than to the presence of glucose per se. 2%. Although the results cited by Fry and Greaves seem to be definitive, other studies indicate that glucose may have effects other than the regulation of moisture content.

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